September 27, 2021
Appraisal of a Simple and Effective RT-qPCR Assay for Evaluating the Reverse Transcriptase Activity in Blood Samples from HIV-1 Patients

Appraisal of a Simple and Effective RT-qPCR Assay for Evaluating the Reverse Transcriptase Activity in Blood Samples from HIV-1 Patients

Testing HIV-1 RNA in plasma by PCR is universally accepted as the last word normal to substantiate prognosis of HIV-1 an infection and to observe viral load in sufferers below therapy. Nevertheless, in some instances, this assay may both underestimate or overestimate the replication capability of a circulating or latent virus. Within the current research, we carried out the evaluation of evaluating the HIV-1 reverse transcriptase (RT) exercise by the use of a brand new assay for the practical screening of the standing of HIV-1 sufferers. To this objective, we utilized, for the primary time on blood samples, an tailored model of a real-time RT quantitative PCR assay, utilized to judge the HIV-1-RT inhibitory exercise of compounds. The research analyzed blood samples from 28 HIV-1-infected sufferers, exhibiting a variety of viremia and immunological values.

Outcomes demonstrated that plasma HIV-1 RT ranges, expressed as cycle threshold values obtained with the assay below appraisal, have been inversely and extremely considerably correlated with the plasma HIV-1-RNA ranges of the sufferers. Thus, an HIV-1 RT quantitative PCR assay was created which we describe on this research, and it might be thought-about as a promising foundation for a further instrument able to furnishing data on the practical virological standing of HIV-1-infected sufferers. Coronavirus Illness (COVID-19) pandemic has affected greater than seven million people in 213 international locations worldwide with a fundamental replica quantity starting from 1.5 to three.5 and an estimated case fatality price starting from 2% to 7%. A considerable proportion of COVID-19 sufferers are asymptomatic; nonetheless, symptomatic instances would possibly current with fever, cough, and dyspnoea or extreme signs as much as acute respiratory misery syndrome.

At present, RNA RT-PCR is the screening instrument, whereas bilateral chest CT is the confirmatory medical diagnostic take a look at. A number of medication have been repurposed to deal with COVID-19, together with chloroquine or hydroxychloroquine with or with out azithromycin, lopinavir/ritonavir mixture, remdesivir, favipiravir, tocilizumab, and EIDD-1931. Lately, Remdesivir gained FDA emergency approval primarily based on promising early findings from the interim evaluation of 1063 sufferers.

 

A delicate and reasonably priced multiplex RT-qPCR assay for SARS-CoV-2 detection

With the continuing COVID-19 (Coronavirus Illness 2019) pandemic, brought on by the novel coronavirus SARS-CoV-2 (Extreme Acute Respiratory Syndrome Coronavirus 2), there’s a want for delicate, particular, and reasonably priced diagnostic exams to establish contaminated people, not all of whom are symptomatic. Essentially the most delicate take a look at entails the detection of viral RNA utilizing RT-qPCR (quantitative reverse transcription PCR), with many industrial kits now out there for this objective. Nevertheless, these are costly, and provide of such kits in adequate numbers can’t all the time be assured.
We subsequently developed a multiplex assay utilizing well-established SARS-CoV-2 targets alongside a human mobile management (RPP30) and a viral spike-in management (Phocine Herpes Virus 1 [PhHV-1]), which monitor pattern high quality and nucleic acid extraction effectivity, respectively. Right here, we set up that this take a look at performs in addition to broadly used industrial assays, however at considerably decreased price.

Moreover, we exhibit >1,000-fold variability in materials routinely collected by mixed nostril and throat swabbing and set up a statistically important correlation between the detected degree of human and SARS-CoV-2 nucleic acids. The inclusion of the human management probe in our assay subsequently offers a quantitative measure of pattern high quality that might assist scale back false-negative charges. We exhibit the feasibility of creating a sturdy RT-qPCR assay at roughly 10% of the price of equal industrial assays, which may gain advantage low-resource environments and make high-volume testing reasonably priced.

 Appraisal of a Simple and Effective RT-qPCR Assay for Evaluating the Reverse Transcriptase Activity in Blood Samples from HIV-1 Patients
Appraisal of a Simple and Effective RT-qPCR Assay for Evaluating the Reverse Transcriptase Activity in Blood Samples from HIV-1 Patients

Detection of Various Maize Chlorotic Mottle Virus Isolates in Maize Seed

Maize chlorotic mottle virus (MCMV) has pushed the emergence of maize deadly necrosis (MLN) worldwide, the place it threatens maize manufacturing in areas of East Africa, South America, and Asia. It’s thought that MCMV transmission by means of seed could also be necessary for introduction of the virus in new areas. Identification of infested seed tons is essential for stopping the unfold of MCMV by means of seed. Though strategies for detecting MCMV in leaf tissue can be found, diagnostic strategies for its detection in seed tons are missing.
On this research, ELISA, RT-PCR, and RT-qPCR have been tailored for detection of MCMV in maize seed. Purified virions of MCMV isolates from Kansas, Mexico and Kenya have been then used to find out the virus detection thresholds for every diagnostic assay. No substantial variations in response have been detected among the many isolates in any of the three assays. The RT-PCR and a SYBRTM green-based RT-qPCR assays have been >3,000 occasions extra delicate than industrial ELISA for MCMV detection. For ELISA utilizing seed extracts, collection of constructive and unfavorable controls was essential, probably due to comparatively excessive backgrounds. Use of seed soak options in ELISA detected MCMV with related sensitivity to seed extracts, produced minimal background, and required considerably much less labor.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

96 WELL BARCODED H1-H12, CLEAR PCR FULL SKIRT PCR AMPLIFICATION MICRO PLATE

PCR-96-FS-C-PBC 10/pk
EUR 168
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 WELL BLACK PCR HALF SKIRT AMPLIFICATION PLATE WITH A SINGLE NOTCH

PCR-96M2-HS-BK 10/pk
EUR 187
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96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE WITH A SINGLE NOTCH

PCR-96M2-HS-C 10/pk
EUR 167
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96 WELL RED PCR HALF SKIRT AMPLIFICATION PLATE WITH A SINGLE NOTCH

PCR-96M2-HS-R 10/pk
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96 WELL YELLOW PCR HALF SKIRT AMPLIFICATION PLATE WITH A SINGLE NOTCH

PCR-96M2-HS-Y 10/pk
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MULTIPLEX KIT PCR MASTITIS PCR kit

PCR-MPX218-48D 50T
EUR 425.8
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MULTIPLEX KIT PCR MASTITIS PCR kit

PCR-MPX218-96D 100T
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MULTIPLEX KIT PCR Babesia & Theileria PCR kit

PCR-MPX401-48D 50T
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MULTIPLEX KIT PCR Babesia & Theileria PCR kit

PCR-MPX401-96D 100T
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Halobacillus PCR kit

PCR-A635-48D 50T
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Halobacillus PCR kit

PCR-A635-96D 100T
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Enterovirus PCR kit

PCR-H433-48R 50T
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Enterovirus PCR kit

PCR-H433-96R 100T
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Mengovirus PCR kit

PCR-H453-48R 50T
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Mengovirus PCR kit

PCR-H453-96R 100T
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Astrovirus PCR kit

PCR-H491-48R 50T
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Astrovirus PCR kit

PCR-H491-96R 100T
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Calicivirus PCR kit

PCR-H493-48R 50T
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Calicivirus PCR kit

PCR-H493-96R 100T
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Adenovirus PCR kit

PCR-H501-48D 50T
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Adenovirus PCR kit

PCR-H501-96D 100T
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Polyomavirus PCR kit

PCR-H515-48D 50T
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Polyomavirus PCR kit

PCR-H515-96D 100T
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Sapovirus PCR kit

PCR-H630-48R 50T
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Sapovirus PCR kit

PCR-H630-96R 100T
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Parechovirus PCR kit

PCR-H642-48R 50T
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PCR-H642-96R 100T
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PCR-H643-48R 50T
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PCR-H643-96R 100T
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Entamoeba PCR kit

PCR-H683-48D 50T
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Entamoeba PCR kit

PCR-H683-96D 100T
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Acanthamoeba PCR kit

PCR-H684-48D 50T
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Acanthamoeba PCR kit

PCR-H684-96D 100T
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Echovirus PCR kit

PCR-H722-48D 50T
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Echovirus PCR kit

PCR-H722-96D 100T
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Description: An conventional PCR kit for detection of Echovirus

ranavirus PCR kit

PCR-V337-48D 50T
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Description: An conventional PCR kit for detection of ranavirus

ranavirus PCR kit

PCR-V337-96D 100T
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Description: An conventional PCR kit for detection of ranavirus

Ruminococcus PCR kit

PCR-V619-48D 50T
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Ruminococcus PCR kit

PCR-V619-96D 100T
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Bifidobacterium PCR kit

PCR-V621-48D 50T
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PCR-V621-96D 100T
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Hantavirus PCR kit

PCR-VH373-48R 50T
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Hantavirus PCR kit

PCR-VH373-96R 100T
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Ruminococcus PCR kit

PCR-H619-48D 48T
EUR 453

Ruminococcus PCR kit

PCR-H619-96D 96T
EUR 572

Bifidobacterium PCR kit

PCR-H621-48D 48T
EUR 453

Bifidobacterium PCR kit

PCR-H621-96D 96T
EUR 572

96 WELL CLEAR PCR HALF SKIRT SINGLE BAR-CODED AMPLIFICATION PLATE WITH A SINGLE NOTCH.

PCR-96M2-HS-C-BC 10/pk
EUR 229
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 WELL BARCODED H1-H12, CLEAR PCR HALF SKIRT AMPLIFICATION PLATE WITH A SINGLE NOTCH

PCR-96M2-HS-C-PBC 10/pk
EUR 227
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

AI M1 gene RT PCR kit

RTq-V603-100R 100T
EUR 776.4
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Description: A Real-Time PCR kit for detection of AI M1 gene .

AI M1 gene RT PCR kit

RTq-V603-150R 150T
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Description: A Real-Time PCR kit for detection of AI M1 gene .

AI M1 gene RT PCR kit

RTq-V603-50R 50T
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Description: A Real-Time PCR kit for detection of AI M1 gene .

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 4941
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PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 4941
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Microthrix parvicella PCR kit

PCR-A575-48D 50T
EUR 425.8
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Microthrix parvicella PCR kit

PCR-A575-96D 100T
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Yersinia pseudotuberculosis PCR kit

PCR-H190-48D 50T
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Yersinia pseudotuberculosis PCR kit

PCR-H190-96D 100T
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Neisseria meningitidis PCR kit

PCR-H421-48D 50T
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Neisseria meningitidis PCR kit

PCR-H421-96D 100T
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Neisseria sp. PCR kit

PCR-H422-48D 50T
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Description: An conventional PCR kit for detection of Neisseria sp.

Neisseria sp. PCR kit

PCR-H422-96D 100T
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Description: An conventional PCR kit for detection of Neisseria sp.

Haemophilus influenzae PCR kit

PCR-H423-48D 50T
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Description: An conventional PCR kit for detection of Haemophilus influenzae

Haemophilus influenzae PCR kit

PCR-H423-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Haemophilus influenzae

Trypanosoma cruzi PCR kit

PCR-H425-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Trypanosoma cruzi

Trypanosoma cruzi PCR kit

PCR-H425-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Trypanosoma cruzi

Cronobacter sakazakii PCR kit

PCR-H431-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Cronobacter sakazakii

Cronobacter sakazakii PCR kit

PCR-H431-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Cronobacter sakazakii

Clostridium botulinum PCR kit

PCR-H432-48D 50T
EUR 425.8
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Clostridium botulinum PCR kit

PCR-H432-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Clostridium botulinum

Virus Zika PCR kit

PCR-H434-48R 50T
EUR 524.4
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Description: An conventional PCR kit for detection of Virus Zika

Virus Zika PCR kit

PCR-H434-96R 100T
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Corynebacterium diphtheriae PCR kit

PCR-H435-48D 50T
EUR 425.8
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Corynebacterium diphtheriae PCR kit

PCR-H435-96D 100T
EUR 521.5
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Legionella pneumophila PCR kit

PCR-H436-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Legionella pneumophila

Legionella pneumophila PCR kit

PCR-H436-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Legionella pneumophila

Bordetella pertussis PCR kit

PCR-H437-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Bordetella pertussis

Bordetella pertussis PCR kit

PCR-H437-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Bordetella pertussis

Bordetella parapertussis PCR kit

PCR-H438-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Bordetella parapertussis

Bordetella parapertussis PCR kit

PCR-H438-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Bordetella parapertussis

Chlamydia pneumoniae PCR kit

PCR-H439-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Chlamydia pneumoniae

Chlamydia pneumoniae PCR kit

PCR-H439-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Chlamydia pneumoniae

Pneumocystis jiroveci PCR kit

PCR-H440-48D 50T
EUR 425.8
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Pneumocystis jiroveci PCR kit

PCR-H440-96D 100T
EUR 521.5
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Actinobacillus actinomycetemcomitans PCR kit

PCR-H446-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Actinobacillus actinomycetemcomitans

Actinobacillus actinomycetemcomitans PCR kit

PCR-H446-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Actinobacillus actinomycetemcomitans

Bacteroides fragilis PCR kit

PCR-H447-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Bacteroides fragilis

Bacteroides fragilis PCR kit

PCR-H447-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Bacteroides fragilis

Candida sp. PCR kit

PCR-H448-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Candida sp.

Candida sp. PCR kit

PCR-H448-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Candida sp.

Enterococcus sp. PCR kit

PCR-H449-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Enterococcus sp.

Enterococcus sp. PCR kit

PCR-H449-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Enterococcus sp.

Neisseria gonorrhoeae PCR kit

PCR-H450-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Neisseria gonorrhoeae

Neisseria gonorrhoeae PCR kit

PCR-H450-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Neisseria gonorrhoeae

Dengue virus PCR kit

PCR-H451-48R 50T
EUR 524.4
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Description: An conventional PCR kit for detection of Dengue virus

Dengue virus PCR kit

PCR-H451-96R 100T
EUR 669.4
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Description: An conventional PCR kit for detection of Dengue virus

Chlamydia trachomatis PCR kit

PCR-H452-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Chlamydia trachomatis

Chlamydia trachomatis PCR kit

PCR-H452-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Chlamydia trachomatis

Human papillomavirus PCR kit

PCR-H454-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Human papillomavirus

Human papillomavirus PCR kit

PCR-H454-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Human papillomavirus

Parvovirus В19 PCR kit

PCR-H455-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Parvovirus В19

Parvovirus В19 PCR kit

PCR-H455-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Parvovirus В19

Treponema pallidum PCR kit

PCR-H456-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Treponema pallidum

Treponema pallidum PCR kit

PCR-H456-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Treponema pallidum

Trichomona vaginalis PCR kit

PCR-H457-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Trichomona vaginalis

Trichomona vaginalis PCR kit

PCR-H457-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Trichomona vaginalis

Ureaplasma parvum PCR kit

PCR-H458-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Ureaplasma parvum

Ureaplasma parvum PCR kit

PCR-H458-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Ureaplasma parvum

Ureaplasma sp PCR kit

PCR-H459-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Ureaplasma sp

Ureaplasma sp PCR kit

PCR-H459-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Ureaplasma sp

Ureaplasma urealyticum PCR kit

PCR-H460-48D 50T
EUR 425.8
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Description: An conventional PCR kit for detection of Ureaplasma urealyticum

Ureaplasma urealyticum PCR kit

PCR-H460-96D 100T
EUR 521.5
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Description: An conventional PCR kit for detection of Ureaplasma urealyticum

Rotavirus A PCR kit

PCR-H480-48R 50T
EUR 524.4
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Description: An conventional PCR kit for detection of Rotavirus A

Rotavirus A PCR kit

PCR-H480-96R 100T
EUR 669.4
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Description: An conventional PCR kit for detection of Rotavirus A

Rubella virus PCR kit

PCR-H481-48R 50T
EUR 524.4
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Description: An conventional PCR kit for detection of Rubella virus

Rubella virus PCR kit

PCR-H481-96R 100T
EUR 669.4
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Description: An conventional PCR kit for detection of Rubella virus

Cryptosporidium parvum PCR kit

PCR-H486-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Cryptosporidium parvum

Cryptosporidium parvum PCR kit

PCR-H486-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Cryptosporidium parvum

Shigella sp. PCR kit

PCR-H488-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Shigella sp.

Shigella sp. PCR kit

PCR-H488-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Shigella sp.

Vibrio cholerae PCR kit

PCR-H489-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Vibrio cholerae

Vibrio cholerae PCR kit

PCR-H489-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Vibrio cholerae

Bacillus cereus PCR kit

PCR-H492-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Bacillus cereus

Bacillus cereus PCR kit

PCR-H492-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Bacillus cereus

Actinomyces israelii PCR kit

PCR-H502-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Actinomyces israelii

Actinomyces israelii PCR kit

PCR-H502-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Actinomyces israelii

Brucella suis PCR kit

PCR-H503-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Brucella suis

Brucella suis PCR kit

PCR-H503-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Brucella suis

Bacillus anthracis PCR kit

PCR-H504-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Bacillus anthracis
ELISA and RT-PCR have been each efficient for detecting MCMV in seed tons from Hawaii and Kenya, with ELISA offering a dependable and cheap diagnostic assay that could possibly be applied routinely in seed testing services.

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