July 17, 2024
Appraisal of a Simple and Effective RT-qPCR Assay for Evaluating the Reverse Transcriptase Activity in Blood Samples from HIV-1 Patients

Appraisal of a Simple and Effective RT-qPCR Assay for Evaluating the Reverse Transcriptase Activity in Blood Samples from HIV-1 Patients

Testing HIV-1 RNA in plasma by PCR is universally accepted as the last word normal to substantiate prognosis of HIV-1 an infection and to observe viral load in sufferers below therapy. Nevertheless, in some instances, this assay may both underestimate or overestimate the replication capability of a circulating or latent virus. Within the current research, we carried out the evaluation of evaluating the HIV-1 reverse transcriptase (RT) exercise by the use of a brand new assay for the practical screening of the standing of HIV-1 sufferers. To this objective, we utilized, for the primary time on blood samples, an tailored model of a real-time RT quantitative PCR assay, utilized to judge the HIV-1-RT inhibitory exercise of compounds. The research analyzed blood samples from 28 HIV-1-infected sufferers, exhibiting a variety of viremia and immunological values.

Outcomes demonstrated that plasma HIV-1 RT ranges, expressed as cycle threshold values obtained with the assay below appraisal, have been inversely and extremely considerably correlated with the plasma HIV-1-RNA ranges of the sufferers. Thus, an HIV-1 RT quantitative PCR assay was created which we describe on this research, and it might be thought-about as a promising foundation for a further instrument able to furnishing data on the practical virological standing of HIV-1-infected sufferers. Coronavirus Illness (COVID-19) pandemic has affected greater than seven million people in 213 international locations worldwide with a fundamental replica quantity starting from 1.5 to three.5 and an estimated case fatality price starting from 2% to 7%. A considerable proportion of COVID-19 sufferers are asymptomatic; nonetheless, symptomatic instances would possibly current with fever, cough, and dyspnoea or extreme signs as much as acute respiratory misery syndrome.

At present, RNA RT-PCR is the screening instrument, whereas bilateral chest CT is the confirmatory medical diagnostic take a look at. A number of medication have been repurposed to deal with COVID-19, together with chloroquine or hydroxychloroquine with or with out azithromycin, lopinavir/ritonavir mixture, remdesivir, favipiravir, tocilizumab, and EIDD-1931. Lately, Remdesivir gained FDA emergency approval primarily based on promising early findings from the interim evaluation of 1063 sufferers.

 

A delicate and reasonably priced multiplex RT-qPCR assay for SARS-CoV-2 detection

With the continuing COVID-19 (Coronavirus Illness 2019) pandemic, brought on by the novel coronavirus SARS-CoV-2 (Extreme Acute Respiratory Syndrome Coronavirus 2), there’s a want for delicate, particular, and reasonably priced diagnostic exams to establish contaminated people, not all of whom are symptomatic. Essentially the most delicate take a look at entails the detection of viral RNA utilizing RT-qPCR (quantitative reverse transcription PCR), with many industrial kits now out there for this objective. Nevertheless, these are costly, and provide of such kits in adequate numbers can’t all the time be assured.
We subsequently developed a multiplex assay utilizing well-established SARS-CoV-2 targets alongside a human mobile management (RPP30) and a viral spike-in management (Phocine Herpes Virus 1 [PhHV-1]), which monitor pattern high quality and nucleic acid extraction effectivity, respectively. Right here, we set up that this take a look at performs in addition to broadly used industrial assays, however at considerably decreased price.

Moreover, we exhibit >1,000-fold variability in materials routinely collected by mixed nostril and throat swabbing and set up a statistically important correlation between the detected degree of human and SARS-CoV-2 nucleic acids. The inclusion of the human management probe in our assay subsequently offers a quantitative measure of pattern high quality that might assist scale back false-negative charges. We exhibit the feasibility of creating a sturdy RT-qPCR assay at roughly 10% of the price of equal industrial assays, which may gain advantage low-resource environments and make high-volume testing reasonably priced.

 Appraisal of a Simple and Effective RT-qPCR Assay for Evaluating the Reverse Transcriptase Activity in Blood Samples from HIV-1 Patients
Appraisal of a Simple and Effective RT-qPCR Assay for Evaluating the Reverse Transcriptase Activity in Blood Samples from HIV-1 Patients

Detection of Various Maize Chlorotic Mottle Virus Isolates in Maize Seed

Maize chlorotic mottle virus (MCMV) has pushed the emergence of maize deadly necrosis (MLN) worldwide, the place it threatens maize manufacturing in areas of East Africa, South America, and Asia. It’s thought that MCMV transmission by means of seed could also be necessary for introduction of the virus in new areas. Identification of infested seed tons is essential for stopping the unfold of MCMV by means of seed. Though strategies for detecting MCMV in leaf tissue can be found, diagnostic strategies for its detection in seed tons are missing.
On this research, ELISA, RT-PCR, and RT-qPCR have been tailored for detection of MCMV in maize seed. Purified virions of MCMV isolates from Kansas, Mexico and Kenya have been then used to find out the virus detection thresholds for every diagnostic assay. No substantial variations in response have been detected among the many isolates in any of the three assays. The RT-PCR and a SYBRTM green-based RT-qPCR assays have been >3,000 occasions extra delicate than industrial ELISA for MCMV detection. For ELISA utilizing seed extracts, collection of constructive and unfavorable controls was essential, probably due to comparatively excessive backgrounds. Use of seed soak options in ELISA detected MCMV with related sensitivity to seed extracts, produced minimal background, and required considerably much less labor.

GenomiPhi HY DNA Amplification Kit 100 rxns - EACH

25660020 EACH
EUR 2346.3

E.coli DNA Amplification Kit in tubes

D415T 1 kit (50 tubes)
EUR 1303.2
Description: E.coli DNA Amplification Kit in tubes by Cygnus Technologies is available in Europe via Gentaur.

E.coli DNA Amplification Kit in wells

D415W 1 kit (96 wells plate)
EUR 1303.2
Description: E.coli DNA Amplification Kit in wells by Cygnus Technologies is available in Europe via Gentaur.

96 WELL BARCODED H1-H12, CLEAR PCR FULL SKIRT PCR AMPLIFICATION MICRO PLATE

PCR-96-FS-C-PBC 10/pk
EUR 201.6
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

GenomiPhi HY DNA Amplification Kit 25 rxns - EACH

25660022 EACH
EUR 757.35

1X Amplification Diluent

H1001-30 30ml
EUR 40
Description: TSA Reagent

1X Amplification Diluent

H1001-60 60ml
EUR 64
Description: TSA Reagent

GenomiPhi HY DNA Amplification Kit 1000 reactions - EACH

25660025 EACH
EUR 22175.1

illustra GenomiPhi V2 DNA Amplification Kit 100 rxns - EACH

25660031 EACH
EUR 606.15

96 WELL RED PCR HALF SKIRT AMPLIFICATION PLATE WITH A SINGLE NOTCH

PCR-96M2-HS-R 10/pk
EUR 234
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Library Amplification SuperMix

20-abx098894
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  • 5 ml

Library Amplification SuperMix

abx098894-100l 100 µl
EUR 737.5

Library Amplification SuperMix

abx098894-200l 200 µl
EUR 1137.5

96 WELL BLACK PCR HALF SKIRT AMPLIFICATION PLATE WITH A SINGLE NOTCH

PCR-96M2-HS-BK 10/pk
EUR 224.4
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE WITH A SINGLE NOTCH

PCR-96M2-HS-C 10/pk
EUR 200.4
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

illustra GenomiPhi V2 DNA Amplification Kit 25 rxns - EACH

25660030 EACH
EUR 224.1

Single Cell Sequence Specific Amplification Kit

P621-01 200 rxn
EUR 435

96 WELL YELLOW PCR HALF SKIRT AMPLIFICATION PLATE WITH A SINGLE NOTCH

PCR-96M2-HS-Y 10/pk
EUR 234
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

illustra GenomiPhi V2 DNA Amplification Kit 500 reactions - EACH

25660032 EACH
EUR 2949.75

illustra TempliPhi 100 Amplification Kit 100 rxns - EACH

25640010 EACH
EUR 305.1

illustra TempliPhi 500 Amplification Kit 500 rxns - EACH

25640050 EACH
EUR 1464.75

Nunc Amplification Tape 96 - PK100

232702 PK100
EUR 257.85

DNA Amplification Kit with Taq DNA Polymerase, 100app

PL1202-K each Ask for price

TruePrime Liquid Biopsy ctDNA Amplification Kit (25 reactions)

EP330025 each
EUR 1057.3

MycoScreen Real-TM NEW Real Time Amplification kit

F23-48FRT 48
EUR 1220.8

VAHTS HiFi Amplification Mix for MGI

NM616-01 24 rxn
EUR 201.6

VAHTS HiFi Amplification Mix for MGI

NM616-02 96 rxn
EUR 363.6

Plasmid amplification and purification

S203 - Ask for price

Coxiella burnetii Real-TM Real Time Amplification kit

B85-50FRT 50
EUR 560.26

DNA Amplification Kit with Max Taq DNA Polymerase, 100app

PL2202-K each Ask for price

DNA Amplification Kit with Chromo Taq DNA Polymerase, 100app

PL1206-K each Ask for price

TruePrime apoptotic cell-free DNA amplification kit (5 Rxn)

EP340005 each
EUR 1139.05

TruePrime apoptotic cell-free DNA amplification kit (20 Rxn)

EP340020 each
EUR 3994.85

HiPer® Random Amplification of Polymorph

HTBM031-5PR 1 unit
EUR 82.29
Description: HiPer® Random Amplification of Polymorph

96 WELL BARCODED H1-H12, CLEAR PCR HALF SKIRT AMPLIFICATION PLATE WITH A SINGLE NOTCH

PCR-96M2-HS-C-PBC 10/pk
EUR 272.4
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

DNA Amplification Kit with Chromo Max Taq DNA Polymerase, 100app

PL2206-K each Ask for price

96 WELL CLEAR PCR HALF SKIRT SINGLE BAR-CODED AMPLIFICATION PLATE WITH A SINGLE NOTCH.

PCR-96M2-HS-C-BC 10/pk
EUR 274.8
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Tyramide Amplification Kit with HRP Streptavidin and Biotin-XX Tyramide

33020-1 KT
EUR 564

Tyramide Amplification Buffer Plus, 1000 reactions

22029-1 KT
EUR 492

Tyramide Amplification Buffer Plus, 250 reactions

22029-T -
EUR 127
Description: N/A

Tyramide Amplification Buffer Plus, 250 reactions

22029-T-1 KT
EUR 127

Tyramide Amplification Kit with HRP Goat Anti-Mouse and Biotin-XX Tyramide

33018-1 KT
EUR 564

VAHTS HiFi Universal Amplification Mix for MGI (SI)

NM618-01 24 rxns
EUR 38.5

VAHTS HiFi Universal Amplification Mix for MGI (SI)

NM618-02 96 rxns
EUR 134

VAHTS HiFi Universal Amplification Mix for MGI (SI)

NM618-00-6rxns 6 rxns
EUR 1.09

VAHTS HiFi Universal Amplification Mix for MGI (SI)

NM618-01-24rxns 24 rxns
EUR 39.87

VAHTS HiFi Universal Amplification Mix for MGI (SI)

NM618-02-96rxns 96 rxns
EUR 138.76

Ready-to-Use Tyramide Amplification Buffer, 1X

9-22027
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  • 100ML
  • 25ML
Description: N/A

Ready-to-Use Tyramide Amplification Buffer, 1X

22027-100mL-1 KT
EUR 472

Ready-to-Use Tyramide Amplification Buffer, 1X

22027-25mL-1 KT
EUR 118

Tyramide Amplification Kit with HRP Streptavidin and CF®543 Tyramide

33005-1 KT
EUR 564

Tyramide Amplification Kit with HRP Streptavidin and CF®568 Tyramide

33008-1 KT
EUR 564

Tyramide Amplification Kit with HRP Streptavidin and CF®594 Tyramide

33011-1 KT
EUR 564

Tyramide Amplification Kit with HRP Goat Anti-Rabbit and Biotin-XX Tyramide

33019-1 KT
EUR 564

Tyramide Amplification Kit with HRP Streptavidin and CF®488A Tyramide

33002-1 KT
EUR 564

Tyramide Amplification Kit with HRP Streptavidin and CF®640R Tyramide

33014-1 KT
EUR 564

Tyramide Amplification Kit with HRP Streptavidin and CF®680R Tyramide

33017-1 KT
EUR 564

Enhancing Wash Buffer for rinse/amplification of I

NB301 4 Liters
EUR 375
Description: Enhancing Wash Buffer for rinse/amplification of IHC-DAB & AEC stains and for fluorescence stains, 4-Liters; Ready-To-Use

Enhancing Wash Buffer for rinse/amplification of I

NB301-5C 1 Liter of 5X
EUR 395
Description: Enhancing Wash Buffer for rinse/amplification of IHC-DAB & AEC stains and for fluorescence stains, 1-Liter of 5X Concentrate

Enhancing Wash Buffer for rinse/amplification of I

NB301S 1 Liter
EUR 155
Description: Enhancing Wash Buffer for rinse/amplification of IHC-DAB & AEC stains and for fluorescence stains, 1-Liter; Ready-To-Use

VAHTS HiFi Universal Amplification Mix for Illumina

N618-01 24 rxns
EUR 38.5

VAHTS HiFi Universal Amplification Mix for Illumina

N618-02 96 rxns
EUR 134

VAHTS HiFi Universal Amplification Mix for Illumina

N618-00-6rxns 6 rxns
EUR 1.09

VAHTS HiFi Universal Amplification Mix for Illumina

N618-01-24rxns 24 rxns
EUR 39.87

VAHTS HiFi Universal Amplification Mix for Illumina

N618-02-96rxns 96 rxns
EUR 138.76

REMBRANDT® HER2 amplification FISH detection assay

C801K.5206 Kit Ask for price

REMBRANDT® HER2 amplification FISH detection assay

C801K.5206.05 Kit
EUR 501

REMBRANDT® HER2 amplification FISH detection assay

C801K.5206.10 Kit
EUR 927

REMBRANDT® TERC amplification FISH detection assay

C814K.2030.05 Kit
EUR 501

REMBRANDT® TERC amplification FISH detection assay

C814K.2030.10 Kit
EUR 927

REMBRANDT® SOX2 amplification FISH detection assay

C815K.2030.05 Kit
EUR 501

REMBRANDT® SOX2 amplification FISH detection assay

C815K.2030.10 Kit
EUR 927

REMBRANDT® EGFR amplification FISH detection assay

C816K.2030.05 Kit
EUR 501

REMBRANDT® EGFR amplification FISH detection assay

C816K.2030.10 Kit
EUR 927

REMBRANDT® MDM2 amplification FISH detection assay

C817K.2030.05 Kit
EUR 501

REMBRANDT® MDM2 amplification FISH detection assay

C817K.2030.10 Kit
EUR 927

Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF®543 Tyramide

33003-1 KT
EUR 564

Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF®568 Tyramide

33006-1 KT
EUR 564

Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF®594 Tyramide

33009-1 KT
EUR 564

Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF®488A Tyramide

33000-1 KT
EUR 564

Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF®543 Tyramide

33004-1 KT
EUR 564

Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF®568 Tyramide

33007-1 KT
EUR 564

Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF®594 Tyramide

33010-1 KT
EUR 564

Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF®640R Tyramide

33012-1 KT
EUR 564

Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF®680R Tyramide

33015-1 KT
EUR 564

Recombinant Adenovirus Amplification (1ml: 1x10E12 VPs/ml)

fAP-0007-1ml Service Ask for price

Recombinant Adenovirus Amplification (3ml: 1x10E12 VPs/ml)

fAP-0007-3ml Service Ask for price

Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF®488A Tyramide

33001-1 KT
EUR 564

Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF®640R Tyramide

33013-1 KT
EUR 564

Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF®680R Tyramide

33016-1 KT
EUR 564

Sa2Res-SAMPLE TO RESULT Fully automated DNA/RNA extractor, PCR setup and qPCR amplification instrument

S2R-48 each Ask for price

REMBRANDT® LSI 3q26.2/CEP3 amplification ISH detection assay

C814K.0199.05 Kit
EUR 501

REMBRANDT® LSI 3q26.2/CEP3 amplification ISH detection assay

C814K.0199.10 Kit
EUR 927

REMBRANDT® LSI 3q26.33/CEP3 amplification ISH detection assay

C815K.0199.05 Kit
EUR 501

REMBRANDT® LSI 3q26.33/CEP3 amplification ISH detection assay

C815K.0199.10 Kit
EUR 927

REMBRANDT® LSI 7p11.2/CEP7 amplification ISH detection assay

C816K.0199.05 Kit
EUR 501

REMBRANDT® LSI 7p11.2/CEP7 amplification ISH detection assay

C816K.0199.10 Kit
EUR 927

REMBRANDT® LSI 17q12/CEP17 amplification ISH detection assay

C801K.0199.05 Kit
EUR 501

REMBRANDT® LSI 17q12/CEP17 amplification ISH detection assay

C801K.0199.10 Kit
EUR 927

REMBRANDT® LSI 12q15/CEP12 amplification ISH detection assay

C817K.0199.05 Kit
EUR 501

REMBRANDT® LSI 12q15/CEP12 amplification ISH detection assay

C817K.0199.10 Kit
EUR 927

Ready-to-go(TM) Genomiphi(TM) V3 DNA Amplification Kit; Cytiva; 25-6601-96; sufficient for 96 reactions - PK96

GE25-6601-96 PK96
EUR 766.8

Phanta UC Super-Fidelity DNA Polymerase for Library Amplification

P507-01 100 U
EUR 188.4

Phanta UC Super-Fidelity DNA Polymerase for Library Amplification

P507-02 500 U
EUR 408

SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)

RA800TC-1 10 reactions
EUR 595

Human Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA810A-1 components)

RA820A-1 20 profiles
EUR 1220

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA812A-1 components)

RA822A-1 20 profiles
EUR 1220

Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit (contains cat# RA800A-1, RA805A-1, RA811A-1 components) 20 profiles

RA821A-1 20 profiles
EUR 1220

Direct qPCR ProbesMasterRobust real-time PCR master mix for amplification directly from blood, swabs or tissue, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-396L 10 x 1,25 ml
EUR 1022
Description: Direct qPCR ProbesMasterRobust real-time PCR master mix for amplification directly from blood, swabs or tissue, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

Direct qPCR ProbesMasterRobust real-time PCR master mix for amplification directly from blood, swabs or tissue, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-396S 2 x 1,25 ml
EUR 256
Description: Direct qPCR ProbesMasterRobust real-time PCR master mix for amplification directly from blood, swabs or tissue, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components)

RA820TC-1 20 profiles
EUR 1533

Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800A-1, RA805A-1, RA810A-1 and EXOTC50A-1 components)

RA821TC-1 20 profiles
EUR 1533

Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)

RA822TC-1 20 profiles
EUR 1533

Direct qPCR ProbesMaster highROXRobust real-time PCR master mix for amplification directly from blood, swabs or tissue with highROX, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-397L 10 x 1,25 ml
EUR 1022
Description: Direct qPCR ProbesMaster highROXRobust real-time PCR master mix for amplification directly from blood, swabs or tissue with highROX, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

Direct qPCR ProbesMaster highROXRobust real-time PCR master mix for amplification directly from blood, swabs or tissue with highROX, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

M-PCR-397S 2 x 1,25 ml
EUR 256
Description: Direct qPCR ProbesMaster highROXRobust real-time PCR master mix for amplification directly from blood, swabs or tissue with highROX, 2 x conc. master mix** shipped ice packs - must be shipped via overnight service

BioLit Animal Tissue Direct PCR Kit (Research)

91210 50 Preps
EUR 222.28
Description: Part E

PCR detection kit for Monkeypox Virus

DA1430 24 test/kit
EUR 129.6
Description: Monkeypox is a viral zoonosis (a virus transmitted to humans from animals) with symptoms very similar to those seen in the past in smallpox patients, although it is clinically less severe. It is caused by the monkeypox virus which belongs to the orthopoxvirus genus of the Poxviridae family. There are two clades of monkeypox virus: the West African clade and the Congo Basin (Central African) clade. The name monkeypox originates from the initial discovery of the virus in monkeys in a Danish laboratory in 1958. The first human case was identified in a child in the Democratic Republic of the Congo in 1970.

BioLit PCR and DNA Fragment Purification Kit (Research)

23512 50 Preps
EUR 94.08
Description: Part E

BioLit PCR and DNA Fragment Purification Kit (Research)

23512-1 100 Preps
EUR 183.54
Description: Part E

AGS8830-8 PCR instrument

AGS8830 1 unit/set Ask for price

BioLit Genomic DNA Extraction Mini Kit (Research)

51826 25 Extractions
EUR 60.55
Description: Part E

BioLit Genomic DNA Extraction Mini Kit (Research)

51826-1 50 Extractions
EUR 117
Description: Part E

BioLit SafeDye Stain

25834 50 mcl
EUR 10.26
Description: Part E

BioLit SafeDye Stain

25834-1 250 mcl
EUR 42.76
Description: Part E

BioLit SafeDye Stain

25834-2 1 ml
EUR 136.84
Description: Part E

BioLit Gel Extraction Kit (Research)

74578 50 Preps
EUR 89.8
Description: Part E

Biolit FluroBronze Stain

50358 50 mcl
EUR 9.75
Description: Part E

Biolit FluroBronze Stain

50358-1 250 mcl
EUR 31.13
Description: Part E

Biolit FluroBronze Stain

50358-2 1 ml
EUR 97.16
Description: Part E

BioLit Plant DNA Extraction Mini Kit (Research)

56860 25 Extractions
EUR 42.76
Description: Part E
ELISA and RT-PCR have been each efficient for detecting MCMV in seed tons from Hawaii and Kenya, with ELISA offering a dependable and cheap diagnostic assay that could possibly be applied routinely in seed testing services.

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