by Testing HIV-1 RNA in plasma by PCR is universally accepted as the last word normal to substantiate prognosis of HIV-1 an infection and to observe viral load in sufferers below therapy. Nevertheless, in some instances, this assay may both underestimate or overestimate the replication capability of a circulating or latent virus. Within the current research, we carried out the evaluation of evaluating the HIV-1 reverse transcriptase (RT) exercise by the use of a brand new assay for the practical screening of the standing of HIV-1 sufferers. To this objective, we utilized, for the primary time on blood samples, an tailored model of a real-time RT quantitative PCR assay, utilized to judge the HIV-1-RT inhibitory exercise of compounds. The research analyzed blood samples from 28 HIV-1-infected sufferers, exhibiting a variety of viremia and immunological values.
Outcomes demonstrated that plasma HIV-1 RT ranges, expressed as cycle threshold values obtained with the assay below appraisal, have been inversely and extremely considerably correlated with the plasma HIV-1-RNA ranges of the sufferers. Thus, an HIV-1 RT quantitative PCR assay was created which we describe on this research, and it might be thought-about as a promising foundation for a further instrument able to furnishing data on the practical virological standing of HIV-1-infected sufferers. Coronavirus Illness (COVID-19) pandemic has affected greater than seven million people in 213 international locations worldwide with a fundamental replica quantity starting from 1.5 to three.5 and an estimated case fatality price starting from 2% to 7%. A considerable proportion of COVID-19 sufferers are asymptomatic; nonetheless, symptomatic instances would possibly current with fever, cough, and dyspnoea or extreme signs as much as acute respiratory misery syndrome.
At present, RNA RT-PCR is the screening instrument, whereas bilateral chest CT is the confirmatory medical diagnostic take a look at. A number of medication have been repurposed to deal with COVID-19, together with chloroquine or hydroxychloroquine with or with out azithromycin, lopinavir/ritonavir mixture, remdesivir, favipiravir, tocilizumab, and EIDD-1931. Lately, Remdesivir gained FDA emergency approval primarily based on promising early findings from the interim evaluation of 1063 sufferers.
A delicate and reasonably priced multiplex RT-qPCR assay for SARS-CoV-2 detection
With the continuing COVID-19 (Coronavirus Illness 2019) pandemic, brought on by the novel coronavirus SARS-CoV-2 (Extreme Acute Respiratory Syndrome Coronavirus 2), there’s a want for delicate, particular, and reasonably priced diagnostic exams to establish contaminated people, not all of whom are symptomatic. Essentially the most delicate take a look at entails the detection of viral RNA utilizing RT-qPCR (quantitative reverse transcription PCR), with many industrial kits now out there for this objective. Nevertheless, these are costly, and provide of such kits in adequate numbers can’t all the time be assured.
We subsequently developed a multiplex assay utilizing well-established SARS-CoV-2 targets alongside a human mobile management (RPP30) and a viral spike-in management (Phocine Herpes Virus 1 [PhHV-1]), which monitor pattern high quality and nucleic acid extraction effectivity, respectively. Right here, we set up that this take a look at performs in addition to broadly used industrial assays, however at considerably decreased price.
Moreover, we exhibit >1,000-fold variability in materials routinely collected by mixed nostril and throat swabbing and set up a statistically important correlation between the detected degree of human and SARS-CoV-2 nucleic acids. The inclusion of the human management probe in our assay subsequently offers a quantitative measure of pattern high quality that might assist scale back false-negative charges. We exhibit the feasibility of creating a sturdy RT-qPCR assay at roughly 10% of the price of equal industrial assays, which may gain advantage low-resource environments and make high-volume testing reasonably priced.
Detection of Various Maize Chlorotic Mottle Virus Isolates in Maize Seed
Maize chlorotic mottle virus (MCMV) has pushed the emergence of maize deadly necrosis (MLN) worldwide, the place it threatens maize manufacturing in areas of East Africa, South America, and Asia. It’s thought that MCMV transmission by means of seed could also be necessary for introduction of the virus in new areas. Identification of infested seed tons is essential for stopping the unfold of MCMV by means of seed. Though strategies for detecting MCMV in leaf tissue can be found, diagnostic strategies for its detection in seed tons are missing.
On this research, ELISA, RT-PCR, and RT-qPCR have been tailored for detection of MCMV in maize seed. Purified virions of MCMV isolates from Kansas, Mexico and Kenya have been then used to find out the virus detection thresholds for every diagnostic assay. No substantial variations in response have been detected among the many isolates in any of the three assays. The RT-PCR and a SYBRTM green-based RT-qPCR assays have been >3,000 occasions extra delicate than industrial ELISA for MCMV detection. For ELISA utilizing seed extracts, collection of constructive and unfavorable controls was essential, probably due to comparatively excessive backgrounds. Use of seed soak options in ELISA detected MCMV with related sensitivity to seed extracts, produced minimal background, and required considerably much less labor.
 PCR Mycoplasma Detection Kit |
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| M034-Kit | TOKU-E | Kit | EUR 266 |
 96 WELL BARCODED H1-H12, CLEAR PCR FULL SKIRT PCR AMPLIFICATION MICRO PLATE |
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| PCR-96-FS-C-PBC | CORNING | 10/pk | EUR 168 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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 96 WELL BLACK PCR HALF SKIRT AMPLIFICATION PLATE WITH A SINGLE NOTCH |
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| PCR-96M2-HS-BK | CORNING | 10/pk | EUR 187 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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 96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE WITH A SINGLE NOTCH |
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| PCR-96M2-HS-C | CORNING | 10/pk | EUR 167 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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 96 WELL RED PCR HALF SKIRT AMPLIFICATION PLATE WITH A SINGLE NOTCH |
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| PCR-96M2-HS-R | CORNING | 10/pk | EUR 195 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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 96 WELL YELLOW PCR HALF SKIRT AMPLIFICATION PLATE WITH A SINGLE NOTCH |
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| PCR-96M2-HS-Y | CORNING | 10/pk | EUR 195 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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 MULTIPLEX KIT PCR MASTITIS PCR kit |
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| PCR-MPX218-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR MASTITIS |
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MULTIPLEX KIT PCR MASTITIS PCR kit |
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| PCR-MPX218-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR MASTITIS |
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 MULTIPLEX KIT PCR Babesia & Theileria PCR kit |
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| PCR-MPX401-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria |
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MULTIPLEX KIT PCR Babesia & Theileria PCR kit |
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| PCR-MPX401-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria |
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 Halobacillus PCR kit |
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| PCR-A635-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Halobacillus |
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Halobacillus PCR kit |
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| PCR-A635-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Halobacillus |
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 Enterovirus PCR kit |
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| PCR-H433-48R | Bioingentech | 50T | EUR 524.4 |
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Description: An conventional PCR kit for detection of Enterovirus |
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Enterovirus PCR kit |
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| PCR-H433-96R | Bioingentech | 100T | EUR 669.4 |
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Description: An conventional PCR kit for detection of Enterovirus |
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 Mengovirus PCR kit |
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| PCR-H453-48R | Bioingentech | 50T | EUR 524.4 |
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Description: An conventional PCR kit for detection of Mengovirus |
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Mengovirus PCR kit |
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| PCR-H453-96R | Bioingentech | 100T | EUR 669.4 |
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Description: An conventional PCR kit for detection of Mengovirus |
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 Astrovirus PCR kit |
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| PCR-H491-48R | Bioingentech | 50T | EUR 524.4 |
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Description: An conventional PCR kit for detection of Astrovirus |
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Astrovirus PCR kit |
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| PCR-H491-96R | Bioingentech | 100T | EUR 669.4 |
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Description: An conventional PCR kit for detection of Astrovirus |
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 Calicivirus PCR kit |
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| PCR-H493-48R | Bioingentech | 50T | EUR 524.4 |
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Description: An conventional PCR kit for detection of Calicivirus |
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Calicivirus PCR kit |
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| PCR-H493-96R | Bioingentech | 100T | EUR 669.4 |
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Description: An conventional PCR kit for detection of Calicivirus |
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 Adenovirus PCR kit |
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| PCR-H501-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Adenovirus |
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Adenovirus PCR kit |
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| PCR-H501-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Adenovirus |
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 Polyomavirus PCR kit |
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| PCR-H515-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Polyomavirus |
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Polyomavirus PCR kit |
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| PCR-H515-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Polyomavirus |
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 Sapovirus PCR kit |
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| PCR-H630-48R | Bioingentech | 50T | EUR 524.4 |
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Description: An conventional PCR kit for detection of Sapovirus |
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Sapovirus PCR kit |
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| PCR-H630-96R | Bioingentech | 100T | EUR 669.4 |
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Description: An conventional PCR kit for detection of Sapovirus |
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 Parechovirus PCR kit |
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| PCR-H642-48R | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Parechovirus |
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Parechovirus PCR kit |
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| PCR-H642-96R | Bioingentech | 100T | EUR 669.4 |
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Description: An conventional PCR kit for detection of Parechovirus |
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 Metapneumovirus PCR kit |
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| PCR-H643-48R | Bioingentech | 50T | EUR 524.4 |
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Description: An conventional PCR kit for detection of Metapneumovirus |
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Metapneumovirus PCR kit |
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| PCR-H643-96R | Bioingentech | 100T | EUR 669.4 |
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Description: An conventional PCR kit for detection of Metapneumovirus |
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 Entamoeba PCR kit |
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| PCR-H683-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Entamoeba |
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Entamoeba PCR kit |
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| PCR-H683-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Entamoeba |
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 Acanthamoeba PCR kit |
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| PCR-H684-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Acanthamoeba |
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Acanthamoeba PCR kit |
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| PCR-H684-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Acanthamoeba |
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 Echovirus PCR kit |
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| PCR-H722-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Echovirus |
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Echovirus PCR kit |
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| PCR-H722-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Echovirus |
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 ranavirus PCR kit |
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| PCR-V337-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of ranavirus |
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ranavirus PCR kit |
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| PCR-V337-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of ranavirus |
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 Ruminococcus PCR kit |
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| PCR-V619-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Ruminococcus |
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Ruminococcus PCR kit |
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| PCR-V619-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Ruminococcus |
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 Bifidobacterium PCR kit |
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| PCR-V621-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Bifidobacterium |
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Bifidobacterium PCR kit |
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| PCR-V621-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Bifidobacterium |
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 Hantavirus PCR kit |
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| PCR-VH373-48R | Bioingentech | 50T | EUR 524.4 |
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Description: An conventional PCR kit for detection of Hantavirus |
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Hantavirus PCR kit |
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| PCR-VH373-96R | Bioingentech | 100T | EUR 669.4 |
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Description: An conventional PCR kit for detection of Hantavirus |
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Ruminococcus PCR kit |
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| PCR-H619-48D | Bioingentech | 48T | EUR 453 |
Ruminococcus PCR kit |
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| PCR-H619-96D | Bioingentech | 96T | EUR 572 |
Bifidobacterium PCR kit |
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| PCR-H621-48D | Bioingentech | 48T | EUR 453 |
Bifidobacterium PCR kit |
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| PCR-H621-96D | Bioingentech | 96T | EUR 572 |
 96 WELL CLEAR PCR HALF SKIRT SINGLE BAR-CODED AMPLIFICATION PLATE WITH A SINGLE NOTCH. |
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| PCR-96M2-HS-C-BC | CORNING | 10/pk | EUR 229 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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 96 WELL BARCODED H1-H12, CLEAR PCR HALF SKIRT AMPLIFICATION PLATE WITH A SINGLE NOTCH |
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| PCR-96M2-HS-C-PBC | CORNING | 10/pk | EUR 227 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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 AI M1 gene RT PCR kit |
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| RTq-V603-100R | Bioingentech | 100T | EUR 776.4 |
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Description: A Real-Time PCR kit for detection of AI M1 gene . |
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AI M1 gene RT PCR kit |
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| RTq-V603-150R | Bioingentech | 150T | EUR 877.9 |
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Description: A Real-Time PCR kit for detection of AI M1 gene . |
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AI M1 gene RT PCR kit |
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| RTq-V603-50R | Bioingentech | 50T | EUR 631.4 |
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Description: A Real-Time PCR kit for detection of AI M1 gene . |
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) PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
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| PIN320A-KIT | SBI | 1 Kit | EUR 4941 |
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) PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
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| PIN340iPS-KIT | SBI | 1 Kit | EUR 4941 |
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 Microthrix parvicella PCR kit |
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| PCR-A575-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Microthrix parvicella |
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Microthrix parvicella PCR kit |
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| PCR-A575-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Microthrix parvicella |
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 Yersinia pseudotuberculosis PCR kit |
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| PCR-H190-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Yersinia pseudotuberculosis |
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Yersinia pseudotuberculosis PCR kit |
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| PCR-H190-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Yersinia pseudotuberculosis |
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 Neisseria meningitidis PCR kit |
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| PCR-H421-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Neisseria meningitidis |
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Neisseria meningitidis PCR kit |
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| PCR-H421-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Neisseria meningitidis |
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 Neisseria sp. PCR kit |
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| PCR-H422-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Neisseria sp. |
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Neisseria sp. PCR kit |
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| PCR-H422-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Neisseria sp. |
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 Haemophilus influenzae PCR kit |
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| PCR-H423-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Haemophilus influenzae |
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Haemophilus influenzae PCR kit |
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| PCR-H423-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Haemophilus influenzae |
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 Trypanosoma cruzi PCR kit |
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| PCR-H425-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Trypanosoma cruzi |
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Trypanosoma cruzi PCR kit |
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| PCR-H425-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Trypanosoma cruzi |
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 Cronobacter sakazakii PCR kit |
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| PCR-H431-48D | Bioingentech | 50T | EUR 425.8 |
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|||
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Description: An conventional PCR kit for detection of Cronobacter sakazakii |
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Cronobacter sakazakii PCR kit |
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| PCR-H431-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Cronobacter sakazakii |
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 Clostridium botulinum PCR kit |
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| PCR-H432-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Clostridium botulinum |
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Clostridium botulinum PCR kit |
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| PCR-H432-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Clostridium botulinum |
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 Virus Zika PCR kit |
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| PCR-H434-48R | Bioingentech | 50T | EUR 524.4 |
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Description: An conventional PCR kit for detection of Virus Zika |
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Virus Zika PCR kit |
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| PCR-H434-96R | Bioingentech | 100T | EUR 669.4 |
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Description: An conventional PCR kit for detection of Virus Zika |
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 Corynebacterium diphtheriae PCR kit |
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| PCR-H435-48D | Bioingentech | 50T | EUR 425.8 |
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|||
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Description: An conventional PCR kit for detection of Corynebacterium diphtheriae |
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Corynebacterium diphtheriae PCR kit |
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| PCR-H435-96D | Bioingentech | 100T | EUR 521.5 |
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|||
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Description: An conventional PCR kit for detection of Corynebacterium diphtheriae |
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 Legionella pneumophila PCR kit |
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| PCR-H436-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Legionella pneumophila |
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Legionella pneumophila PCR kit |
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| PCR-H436-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Legionella pneumophila |
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 Bordetella pertussis PCR kit |
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| PCR-H437-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of Bordetella pertussis |
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Bordetella pertussis PCR kit |
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| PCR-H437-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of Bordetella pertussis |
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 Bordetella parapertussis PCR kit |
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| PCR-H438-48D | Bioingentech | 50T | EUR 425.8 |
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|||
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Description: An conventional PCR kit for detection of Bordetella parapertussis |
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Bordetella parapertussis PCR kit |
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| PCR-H438-96D | Bioingentech | 100T | EUR 521.5 |
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|||
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Description: An conventional PCR kit for detection of Bordetella parapertussis |
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 Chlamydia pneumoniae PCR kit |
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| PCR-H439-48D | Bioingentech | 50T | EUR 425.8 |
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|||
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Description: An conventional PCR kit for detection of Chlamydia pneumoniae |
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Chlamydia pneumoniae PCR kit |
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| PCR-H439-96D | Bioingentech | 100T | EUR 521.5 |
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|||
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Description: An conventional PCR kit for detection of Chlamydia pneumoniae |
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 Pneumocystis jiroveci PCR kit |
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| PCR-H440-48D | Bioingentech | 50T | EUR 425.8 |
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|||
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Description: An conventional PCR kit for detection of Pneumocystis jiroveci |
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Pneumocystis jiroveci PCR kit |
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| PCR-H440-96D | Bioingentech | 100T | EUR 521.5 |
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|||
|
Description: An conventional PCR kit for detection of Pneumocystis jiroveci |
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 Actinobacillus actinomycetemcomitans PCR kit |
|||
| PCR-H446-48D | Bioingentech | 50T | EUR 425.8 |
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|||
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Description: An conventional PCR kit for detection of Actinobacillus actinomycetemcomitans |
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Actinobacillus actinomycetemcomitans PCR kit |
|||
| PCR-H446-96D | Bioingentech | 100T | EUR 521.5 |
|
|||
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Description: An conventional PCR kit for detection of Actinobacillus actinomycetemcomitans |
|||
 Bacteroides fragilis PCR kit |
|||
| PCR-H447-48D | Bioingentech | 50T | EUR 425.8 |
|
|||
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Description: An conventional PCR kit for detection of Bacteroides fragilis |
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Bacteroides fragilis PCR kit |
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| PCR-H447-96D | Bioingentech | 100T | EUR 521.5 |
|
|||
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Description: An conventional PCR kit for detection of Bacteroides fragilis |
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 Candida sp. PCR kit |
|||
| PCR-H448-48D | Bioingentech | 50T | EUR 425.8 |
|
|||
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Description: An conventional PCR kit for detection of Candida sp. |
|||
Candida sp. PCR kit |
|||
| PCR-H448-96D | Bioingentech | 100T | EUR 521.5 |
|
|||
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Description: An conventional PCR kit for detection of Candida sp. |
|||
 Enterococcus sp. PCR kit |
|||
| PCR-H449-48D | Bioingentech | 50T | EUR 425.8 |
|
|||
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Description: An conventional PCR kit for detection of Enterococcus sp. |
|||
Enterococcus sp. PCR kit |
|||
| PCR-H449-96D | Bioingentech | 100T | EUR 521.5 |
|
|||
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Description: An conventional PCR kit for detection of Enterococcus sp. |
|||
 Neisseria gonorrhoeae PCR kit |
|||
| PCR-H450-48D | Bioingentech | 50T | EUR 425.8 |
|
|||
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Description: An conventional PCR kit for detection of Neisseria gonorrhoeae |
|||
Neisseria gonorrhoeae PCR kit |
|||
| PCR-H450-96D | Bioingentech | 100T | EUR 521.5 |
|
|||
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Description: An conventional PCR kit for detection of Neisseria gonorrhoeae |
|||
 Dengue virus PCR kit |
|||
| PCR-H451-48R | Bioingentech | 50T | EUR 524.4 |
|
|||
|
Description: An conventional PCR kit for detection of Dengue virus |
|||
ELISA and RT-PCR have been each efficient for detecting MCMV in seed tons from Hawaii and Kenya, with ELISA offering a dependable and cheap diagnostic assay that could possibly be applied routinely in seed testing services.
